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Mylène ZARKA
mylene.zarka@inserm.fr

Hôpital Lariboisière
2, rue Ambroise Paré
75457 Paris Cedex 10

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Histology

The BIOSCAR histology core facility specialises in the processing and analysis of mineralised tissues.
It is open to various teams on site as well as to external teams, whether from institutions or the private sector. All equipment is available for use, either on a loan basis with staff training or as a service provided on request following a project assessment. The platform is committed to providing a high-quality service combined with personalised support throughout the implementation of the project.

/ Expertises

1. Non-decalcified hard tissues study (embedding in methyl methacrylate resin) :

  • Quantification of static bone parameters (labelling or staining of osteoblasts, osteoclasts and osteocytes)
  • Quantification of dynamic formation parameters
  • Analysis of the bone matrix and its state of mineralisation
  • Preparation of thick sections, for example for the analysis of microcracks

2. Decalcified hard tissues or soft tissues study (embedding in paraffin or OCT) :

  • Quantification of cell proliferation/survival parameters (apoptosis of bone cells, proliferation)
  • Study of vascularisation and the medullary microenvironment via immunolabelling
  • Characterisation of bone cells and their differentiation states via immunolabelling
  • Histology can also be combined with imaging analysis offered on our other dedicated platform, including for measurements of bone density and morphology

/ Services offered

  1. Sample preparation (fixation, embedding)
  2. Sectioning of samples (paraffin, resin, low temperature)
  3. Staining
  4. Immunohistochemistry
  5. Bone histomorphometric analysis with analysis of static and dynamic parameters
  6. All analyses specific to non-decalcified hard tissues, on request
(All services are upon request and following a review of the project)

/ Equipment

Sample preparation

  • 1 paraffin embedding machine
  • 1 MM France EC500 paraffin embedding platform

Sample sectioning

  • 2 resin microtomes (Leica SM2500 and Leica SM2500S)
  • 1 ThermoScientific NX70 cryostat
  • 2 paraffin microtomes (Leica RM2255 and Leica Autocut)
  • 1 Q ATM QPOL 250 M1 polisher
  • 1 Buehler Low Speed Saw isometer

Image analysis

  • 2 optical microscopy stations coupled with Microvision Instruments image analysers (Nikon Optiphot2 and Nikon Eclipse 50 with polarised light)
  • 1 optical microscopy station with a motorised stage coupled with a Microvision Instruments touchscreen and stylus image analyser (Zeiss AxioImager)
  • 1 fluorescence microscope with optical sectioning via Zeiss Apotome structured illumination (DAPI, FITC, dsRed, Cy5)
  • 1 Zeiss LSM800 confocal microscope (DAPI, FITC, Rhodamine filter cube) with motorised stage and 405, 488, 561 and 640 nm lasers

/ Faculty

Mylène ZARKA
Engineer
mylene.zarka@inserm.fr

Agnès OSTERTAG – Engineer researcher
Morgane BOURMAUD – Engineer
Nathalie ANDROCLES – Engineer assistant
Pascale CHANTRENNE – Technician
Jacqueline FRANCISQUIN – Technician
Gwenaelle JAYAT – Technician
Maeva ROULLAT – Technician
Sylvie THOMASSEAU – Technician

/ Selected Publications

View all our publications

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Feb 2016 – ID : Care Res (Hoboken), 68(2):261-6.
Bardin T, Bouée S, Clerson P, Chalès G, Flipo RM, Lioté F, Perez V, Poiraud T, Schaeverbeke T, Richette P
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May 2016 – ID : Clin. Genet. 89 ; 746-8
Bardin T, Chalès G, Pascart T, Flipo RM, Korng Ea H, Roujeau JC, Delayen A, Clerson P.
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May 2017 – ID : Ann Rheum Dis, 76(5):811-820
Bardin T, Keenan RT, Khanna PP, Kopicko J, Fung M, Bhakta N, Adler S, Storgard C, Baumgartner S, So A
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